Towards single-cell genome analysis of circulating tumor cells based on microcavity array

Tomoko Yoshino, Hisashige Kanbara, Ryo Negishi, Kaori Takai, Tadashi Matsunaga, Tsuyoshi Tanaka

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

Circulating tumor cells (CTCs) are defined as tumor cells circulating in the peripheral blood of patients with metastatic cancer. CTCs are well recognized as accessible and valuable biomarkers for cancer diagnosis and are a potential target for drug discovery. However, the recovery of such extremely rare CTCs from the peripheral blood, which contains a mixture of CTCs and a large number of hematologic cells, presents a technical challenge. In this study, techniques for the recovery of CTCs based on the microcavity array (MCA) are introduced. The MCA can specifically obtain tumor cells from whole blood based on differences in the size and deformability between tumor cells and hematologic cells. By controlling the size and shape of the cavity, efficient CTC recovery was achieved in our study. Furthermore, an automated CTC recovery system using a MCA was developed for subsequent high-throughput analysis. The MCA system was finally applied to single-cell genome analysis, and accurate single-cell manipulation and genome amplification without any contamination of neighboring cells and without loss of target cells was achieved.

本文言語English
ホスト出版物のタイトル2016 World Automation Congress, WAC 2016
出版社IEEE Computer Society
2016-October
ISBN(電子版)9781889335513
DOI
出版ステータスPublished - 2016 10 4
外部発表はい
イベント2016 World Automation Congress, WAC 2016 - Rio Grande, United States
継続期間: 2016 7 312016 8 4

Other

Other2016 World Automation Congress, WAC 2016
CountryUnited States
CityRio Grande
Period16/7/3116/8/4

ASJC Scopus subject areas

  • Control and Systems Engineering

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