Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Liming Xu, Yoshikazu Kurusu, Kaori Takizawa, Junzo Tanaka, Kojiro Matsumoto, Akiyoshi Taniguchi*

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

本文言語English
ページ(範囲)1070-1074
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
307
4
DOI
出版ステータスPublished - 2003 8 8
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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