Transcriptional regulation of human Galβ1,3GalNAc/Galβ1, 4GlcNAc α2,3-sialyltransferase (hST3Gal IV) gene in testis and ovary cell lines

Akiyoshi Taniguchi*, Maiko Hioki, Kojiro Matsumoto

*この研究の対応する著者

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The mRNA expression of sialyltransferase genes is regulated in a cell-type-specific manner. The mRNAs of human Galβ1, 3GalNAc/Galβ1, 4 GlcNAc α2,3-sialyltransferase gene (hST3Gal IV) consist of six isoforms, type A1, A2, B1, B2, B3, and BX. These mRNAs are transcribed from different promoters, pA, pB1, pB2, pB3, and pBX, respectively. Type B mRNAs are expressed in several cells, whereas type A mRNAs are specifically expressed in testis, ovary, and placenta, suggesting that pA promoter activity is especially high in these tissues. We show herein germ-cell specific transcriptional regulation of the hST3Gal IV pA promoter. Using a luciferase assay, pA promoter activity is shown to be high in testis and ovary cell lines. We identified the enhancer region of the pA promoter, located at nt -520 to -420. These results suggest that this element plays a critical role in germ-cell specific regulation of the pA promoter. The results of site-directed mutagenesis suggest that AP2 and c-Ets sites in this region are involved in pA promoter activity, which in turn suggests that the hST3Gal IV gene is regulated in a tissue-restricted fashion at the level of transcription.

本文言語English
ページ(範囲)764-768
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
301
3
DOI
出版ステータスPublished - 2003 2 14
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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