Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli

Namthip Chumpolkulwong, Kensaku Sakamoto, Akiko Hayashi, Fumie Iraha, Naoko Shinya, Natsuko Matsuda, Daisuke Kiga, Akiko Urushibata, Mikako Shirouzu, Kenji Oki, Takanori Kigawa, Shigeyuki Yokoyama

研究成果: Article

22 引用 (Scopus)

抜粋

We analyzed the effect of nine 'rare' codons (AGA, AGG, AUA, CCC, CGA, CGG, CUA, GGA, and UUA) on gene expression in an Escherichia coli coupled transcription/translation cell-free system, in comparison with a cell-based expression system. Each reporter gene contained five consecutive repeats of a rare codon, or in some experiments, three consecutive repeats. The cell-free expression of the genes bearing the codons CGA, CUA, GGA, and UUA was not affected, although these codons, except for GGA, were inefficiently translated in E. coli cells. Translation of the remaining five codons (AGA, AGG, AUA, CCC, and CGG) was severely reduced in both systems, and was remarkably facilitated in the cell-free system based on an S30 extract from the E. coli cells overproducing 'minor' tRNAs for these codons.

元の言語English
ページ(範囲)31-36
ページ数6
ジャーナルJournal of structural and functional genomics
7
発行部数1
DOI
出版物ステータスPublished - 2006 3 1
外部発表Yes

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

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  • これを引用

    Chumpolkulwong, N., Sakamoto, K., Hayashi, A., Iraha, F., Shinya, N., Matsuda, N., Kiga, D., Urushibata, A., Shirouzu, M., Oki, K., Kigawa, T., & Yokoyama, S. (2006). Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli. Journal of structural and functional genomics, 7(1), 31-36. https://doi.org/10.1007/s10969-006-9007-y