Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli

Namthip Chumpolkulwong, Kensaku Sakamoto, Akiko Hayashi, Fumie Iraha, Naoko Shinya, Natsuko Matsuda, Daisuke Kiga, Akiko Urushibata, Mikako Shirouzu, Kenji Oki, Takanori Kigawa, Shigeyuki Yokoyama*

*この研究の対応する著者

研究成果: Article査読

23 被引用数 (Scopus)

抄録

We analyzed the effect of nine 'rare' codons (AGA, AGG, AUA, CCC, CGA, CGG, CUA, GGA, and UUA) on gene expression in an Escherichia coli coupled transcription/translation cell-free system, in comparison with a cell-based expression system. Each reporter gene contained five consecutive repeats of a rare codon, or in some experiments, three consecutive repeats. The cell-free expression of the genes bearing the codons CGA, CUA, GGA, and UUA was not affected, although these codons, except for GGA, were inefficiently translated in E. coli cells. Translation of the remaining five codons (AGA, AGG, AUA, CCC, and CGG) was severely reduced in both systems, and was remarkably facilitated in the cell-free system based on an S30 extract from the E. coli cells overproducing 'minor' tRNAs for these codons.

本文言語English
ページ(範囲)31-36
ページ数6
ジャーナルJournal of structural and functional genomics
7
1
DOI
出版ステータスPublished - 2006 3月
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生化学
  • 遺伝学

フィンガープリント

「Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル