Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells

Shigehiko Yumura, Taro Q.P. Uyeda

研究成果: Article査読

65 被引用数 (Scopus)

抄録

Fluorescently labeled myosin moved and accumulated circumferentially in the equatorial region of dividing Dictyostelium cells within a time course of 4 min, followed by contraction of the contractile ring. To investigate the mechanism of this transport process, we have expressed three mutant myosins that cannot hydrolyze ATP in myosin null cells. Immunofluorescence staining showed that these mutant myosins were also correctly transported to the equatorial region, although no contraction followed. The rates of transport, measured using green fluorescent protein-fused myosins, were indistinguishable between wild-type and mutant myosins. These observations demonstrate that myosin is passively transported toward the equatorial region and incorporated into the forming contractile ring without its own motor activity.

本文言語English
ページ(範囲)2089-2099
ページ数11
ジャーナルMolecular biology of the cell
8
10
DOI
出版ステータスPublished - 1997
外部発表はい

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

フィンガープリント 「Transport of myosin II to the equatorial region without its own motor activity in mitotic Dictyostelium cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル