Trapping of biological macromolecules in the three-dimensional mesocage pore cavities of monolith adsorbents

M. A. Shenashen, Sherif A. El-Safty, M. Khairy

    研究成果: Article

    22 引用 (Scopus)

    抄録

    Gene technology is experiencing remarkable progress, and proteins are becoming crucial in the field of disease diagnosis and treatment. Adsorption of biomolecules on the surface of inorganic materials is an important technique for diagnostic assays and gene applications. In this study, highly ordered mesocage cubic Pm3n aluminumsilica monoliths were fabricated by the one-pot direct-templating of a microemulsion of the liquid crystalline phases of a Brij 56 surfactant. Mesocage cubic Pm3n aluminosilica monoliths with well-defined mesostructures offer high adsorption and loading capacity of proteins from an aqueous solution. Three-dimensional monoliths characterized by spherical pore cavities can potentially perform efficient adsorption and trapping of insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobin proteins. A wide variety of characterization techniques such as SAXS, SEM, TEM, the Brunauer-Emmett- Teller method for nitrogen adsorption and surface area measurements, and TEM were used. The adsorption of proteins as well as the kinetic and thermodynamic characteristics of adsorption was studied, and adsorption isotherms were described by the Langmuir equation. Our findings indicated that monolayer coverage of proteins formed on mesoporous adsorbent surfaces during immobilization and uptake assays. Adsorption efficiency of proteins was attained after a number of reuse cycles, which indicates the presence of mesoporous adsorbents of biomolecules. Integration of mesoporous adsorbents may be feasible in various scientific fields such as nanobioscience, material science, artificial implantation, protein purification, biosensors, drug delivery systems, and molecular biology/biotechnology.

    元の言語English
    ページ(範囲)679-692
    ページ数14
    ジャーナルJournal of Porous Materials
    20
    発行部数4
    DOI
    出版物ステータスPublished - 2013 8

    Fingerprint

    Macromolecules
    Adsorbents
    Proteins
    Adsorption
    Biomolecules
    Assays
    Cetomacrogol
    Genes
    Transmission electron microscopy
    Molecular biology
    Myoglobin
    Microemulsions
    Materials science
    Cytochromes
    Biotechnology
    Muramidase
    Adsorption isotherms
    Insulin
    Biosensors
    Surface-Active Agents

    ASJC Scopus subject areas

    • Mechanical Engineering
    • Mechanics of Materials
    • Materials Science(all)

    これを引用

    Trapping of biological macromolecules in the three-dimensional mesocage pore cavities of monolith adsorbents. / Shenashen, M. A.; El-Safty, Sherif A.; Khairy, M.

    :: Journal of Porous Materials, 巻 20, 番号 4, 08.2013, p. 679-692.

    研究成果: Article

    Shenashen, M. A. ; El-Safty, Sherif A. ; Khairy, M. / Trapping of biological macromolecules in the three-dimensional mesocage pore cavities of monolith adsorbents. :: Journal of Porous Materials. 2013 ; 巻 20, 番号 4. pp. 679-692.
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    abstract = "Gene technology is experiencing remarkable progress, and proteins are becoming crucial in the field of disease diagnosis and treatment. Adsorption of biomolecules on the surface of inorganic materials is an important technique for diagnostic assays and gene applications. In this study, highly ordered mesocage cubic Pm3n aluminumsilica monoliths were fabricated by the one-pot direct-templating of a microemulsion of the liquid crystalline phases of a Brij 56 surfactant. Mesocage cubic Pm3n aluminosilica monoliths with well-defined mesostructures offer high adsorption and loading capacity of proteins from an aqueous solution. Three-dimensional monoliths characterized by spherical pore cavities can potentially perform efficient adsorption and trapping of insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobin proteins. A wide variety of characterization techniques such as SAXS, SEM, TEM, the Brunauer-Emmett- Teller method for nitrogen adsorption and surface area measurements, and TEM were used. The adsorption of proteins as well as the kinetic and thermodynamic characteristics of adsorption was studied, and adsorption isotherms were described by the Langmuir equation. Our findings indicated that monolayer coverage of proteins formed on mesoporous adsorbent surfaces during immobilization and uptake assays. Adsorption efficiency of proteins was attained after a number of reuse cycles, which indicates the presence of mesoporous adsorbents of biomolecules. Integration of mesoporous adsorbents may be feasible in various scientific fields such as nanobioscience, material science, artificial implantation, protein purification, biosensors, drug delivery systems, and molecular biology/biotechnology.",
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    AB - Gene technology is experiencing remarkable progress, and proteins are becoming crucial in the field of disease diagnosis and treatment. Adsorption of biomolecules on the surface of inorganic materials is an important technique for diagnostic assays and gene applications. In this study, highly ordered mesocage cubic Pm3n aluminumsilica monoliths were fabricated by the one-pot direct-templating of a microemulsion of the liquid crystalline phases of a Brij 56 surfactant. Mesocage cubic Pm3n aluminosilica monoliths with well-defined mesostructures offer high adsorption and loading capacity of proteins from an aqueous solution. Three-dimensional monoliths characterized by spherical pore cavities can potentially perform efficient adsorption and trapping of insulin, cytochrome C, lysozyme, myoglobin, β-lactoglobin proteins. A wide variety of characterization techniques such as SAXS, SEM, TEM, the Brunauer-Emmett- Teller method for nitrogen adsorption and surface area measurements, and TEM were used. The adsorption of proteins as well as the kinetic and thermodynamic characteristics of adsorption was studied, and adsorption isotherms were described by the Langmuir equation. Our findings indicated that monolayer coverage of proteins formed on mesoporous adsorbent surfaces during immobilization and uptake assays. Adsorption efficiency of proteins was attained after a number of reuse cycles, which indicates the presence of mesoporous adsorbents of biomolecules. Integration of mesoporous adsorbents may be feasible in various scientific fields such as nanobioscience, material science, artificial implantation, protein purification, biosensors, drug delivery systems, and molecular biology/biotechnology.

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