Two nucleotides 5'-adjacent to the anticodon of rat cytoplasmic tRNA(Asp) are not edited

K. Tomita, T. Ueda*, K. Watanabe

*この研究の対応する著者

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Cytoplasmic tRNA(Asp) of rat liver was purified by the solid-phase hybridization method and its nucleotide sequence was analyzed by Donis-Keller's method. The results suggested that the two nucleotides next to the anticodon were identical to its gene sequence, a finding that is inconsistent with a previous report demonstrating by several methods that C32 and T33 on the tRNA(Asp) gene are post-transcriptionally converted to U32 and C33, respectively. Our results indicate that the tRNA hybridized to an oligonucleotide, designed on the basis of the tRNA(Asp) gene sequence, undergoes no editing and possesses C32 and U33 as predicted from the DNA sequence. Analysis of cDNA synthesized from the purified tRNA(Asp) by the RT-PCR method supported the finding that RNA editing is not involved in the maturation process of rat cytoplasmic tRNA(Asp).

本文言語English
ページ(範囲)1001-1006
ページ数6
ジャーナルBiochimie
78
11-12
DOI
出版ステータスPublished - 1996 1 1
外部発表はい

ASJC Scopus subject areas

  • 生化学

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