Two nucleotides 5'-adjacent to the anticodon of rat cytoplasmic tRNA(Asp) are not edited

K. Tomita, T. Ueda, K. Watanabe

研究成果: Article

2 引用 (Scopus)

抜粋

Cytoplasmic tRNA(Asp) of rat liver was purified by the solid-phase hybridization method and its nucleotide sequence was analyzed by Donis-Keller's method. The results suggested that the two nucleotides next to the anticodon were identical to its gene sequence, a finding that is inconsistent with a previous report demonstrating by several methods that C32 and T33 on the tRNA(Asp) gene are post-transcriptionally converted to U32 and C33, respectively. Our results indicate that the tRNA hybridized to an oligonucleotide, designed on the basis of the tRNA(Asp) gene sequence, undergoes no editing and possesses C32 and U33 as predicted from the DNA sequence. Analysis of cDNA synthesized from the purified tRNA(Asp) by the RT-PCR method supported the finding that RNA editing is not involved in the maturation process of rat cytoplasmic tRNA(Asp).

元の言語English
ページ(範囲)1001-1006
ページ数6
ジャーナルBiochimie
78
発行部数11-12
DOI
出版物ステータスPublished - 1996 1 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry

フィンガープリント Two nucleotides 5'-adjacent to the anticodon of rat cytoplasmic tRNA(Asp) are not edited' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用