Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector

Y. Hirowatari, M. Hijikata, Y. Tanji, H. Nyunoya, H. Mizushima, K. Kimura, T. Tanaka, N. Kato, K. Shimotohno

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P 1 and P 1′ of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.

本文言語English
ページ(範囲)349-356
ページ数8
ジャーナルArchives of Virology
133
3-4
DOI
出版ステータスPublished - 1993 9 1
外部発表はい

ASJC Scopus subject areas

  • Virology

フィンガープリント 「Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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