Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector

Y. Hirowatari, M. Hijikata, Y. Tanji, H. Nyunoya, H. Mizushima, K. Kimura, T. Tanaka, N. Kato, K. Shimotohno

研究成果: Article

37 引用 (Scopus)

抜粋

Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P 1 and P 1′ of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.

元の言語English
ページ(範囲)349-356
ページ数8
ジャーナルArchives of Virology
133
発行部数3-4
DOI
出版物ステータスPublished - 1993 9 1
外部発表Yes

ASJC Scopus subject areas

  • Virology

フィンガープリント Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Hirowatari, Y., Hijikata, M., Tanji, Y., Nyunoya, H., Mizushima, H., Kimura, K., Tanaka, T., Kato, N., & Shimotohno, K. (1993). Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector. Archives of Virology, 133(3-4), 349-356. https://doi.org/10.1007/BF01313774