抄録
Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P 1 and P 1′ of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.
本文言語 | English |
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ページ(範囲) | 349-356 |
ページ数 | 8 |
ジャーナル | Archives of Virology |
巻 | 133 |
号 | 3-4 |
DOI | |
出版ステータス | Published - 1993 9月 |
外部発表 | はい |
ASJC Scopus subject areas
- ウイルス学