Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase

Shuichi Hirabayashi, Yasuhiko Matsushita, Michio Sato, Rie Oh-I, Masahiro Kasahara, Hiroshi Abe, Hiroshi Nyunoya

研究成果: Article査読

11 被引用数 (Scopus)

抄録

A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.

本文言語English
ページ(範囲)35-45
ページ数11
ジャーナルPlant Biotechnology
21
1
DOI
出版ステータスPublished - 2004 3
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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