Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase

Shuichi Hirabayashi, Yasuhiko Matsushita, Michio Sato, Rie Oh-I, Masahiro Kasahara, Hiroshi Abe, Hiroshi Nyunoya

研究成果: Article

9 引用 (Scopus)

抄録

A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.

元の言語English
ページ(範囲)35-45
ページ数11
ジャーナルPlant Biotechnology
21
発行部数1
出版物ステータスPublished - 2004 3
外部発表Yes

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Proton Pumps
proton pump
Brassinosteroids
Gene Library
cDNA libraries
phosphorylation
phosphotransferases (kinases)
Phosphotransferases
brassinosteroids
Phosphorylation
screening
rice
receptors
Clone Cells
clones
brassinolide
Proteins
H-transporting ATP synthase
Proton-Translocating ATPases
proteins

ASJC Scopus subject areas

  • Plant Science

これを引用

Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase. / Hirabayashi, Shuichi; Matsushita, Yasuhiko; Sato, Michio; Oh-I, Rie; Kasahara, Masahiro; Abe, Hiroshi; Nyunoya, Hiroshi.

:: Plant Biotechnology, 巻 21, 番号 1, 03.2004, p. 35-45.

研究成果: Article

Hirabayashi, Shuichi ; Matsushita, Yasuhiko ; Sato, Michio ; Oh-I, Rie ; Kasahara, Masahiro ; Abe, Hiroshi ; Nyunoya, Hiroshi. / Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase. :: Plant Biotechnology. 2004 ; 巻 21, 番号 1. pp. 35-45.
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AU - Hirabayashi, Shuichi

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AU - Sato, Michio

AU - Oh-I, Rie

AU - Kasahara, Masahiro

AU - Abe, Hiroshi

AU - Nyunoya, Hiroshi

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