抄録
A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 35-45 |
| ページ数 | 11 |
| ジャーナル | Plant Biotechnology |
| 巻 | 21 |
| 発行部数 | 1 |
| 出版物ステータス | Published - 2004 3 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Plant Science
これを引用
Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase. / Hirabayashi, Shuichi; Matsushita, Yasuhiko; Sato, Michio; Oh-I, Rie; Kasahara, Masahiro; Abe, Hiroshi; Nyunoya, Hiroshi.
：: Plant Biotechnology, 巻 21, 番号 1, 03.2004, p. 35-45.研究成果: Article
}
TY - JOUR
T1 - Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase
AU - Hirabayashi, Shuichi
AU - Matsushita, Yasuhiko
AU - Sato, Michio
AU - Oh-I, Rie
AU - Kasahara, Masahiro
AU - Abe, Hiroshi
AU - Nyunoya, Hiroshi
PY - 2004/3
Y1 - 2004/3
N2 - A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.
AB - A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.
KW - Brassinosteroid
KW - BRI1
KW - H-ATPase
KW - Lamina joint
KW - Phosphorylation screening
KW - Protein interaction
KW - Proton pump interactor
KW - Receptor kinase
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=3042848685&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042848685&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:3042848685
VL - 21
SP - 35
EP - 45
JO - Plant Biotechnology
JF - Plant Biotechnology
SN - 1342-4580
IS - 1
ER -