抄録
A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.
本文言語 | English |
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ページ(範囲) | 35-45 |
ページ数 | 11 |
ジャーナル | Plant Biotechnology |
巻 | 21 |
号 | 1 |
DOI | |
出版ステータス | Published - 2004 3月 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- 農業および作物学
- 植物科学