Two types of coagulogen mRNAs found in horseshoe crab (Tachypleus tridentatus) hemocytes: Molecular cloning and nucleotide sequences

Toshiyuki Miyata, Hiroaki Matsumoto, Masahira Hattori, Yoshiyuki Sakaki, Sadaaki Iwanaga

研究成果: Article

9 引用 (Scopus)

抄録

The complete cDNA sequence coding for the coagulogen present in the horseshoe crab (Tachypleus tridentatus) hemocytes was determined. Clones carrying cDNA fragments for coagulogen were isolated from a cDNA library of the hemocyte mRNA using synthetic oligodeoxyribonuclcotides as probes. The nucleotide sequence analyses of the cloned cDNAs revealed that the hemocyte coagulogen consists of 175 amino acids with 20 amino acids in a presegment, and that there are two types of mRNAs for coagulogen. The two mRNAs exhibited three nucleotide substitutions, two of which were in their protein-coding regions, resulting in two amino acid replacements. Subsequently, two molecular species of coagulogen, named coagulogens type I and type II, were identified by tryptic peptide mapping of the mature proteins isolated from the hemocyte lysate. These results suggest that the two types of coagulogens are first synthesized as preproteins and are incorporated into the granules that are abundantly present in the hemocytes with liberation of the signal peptides.

元の言語English
ページ(範囲)213-220
ページ数8
ジャーナルJournal of Biochemistry
100
発行部数1
出版物ステータスPublished - 1986 7
外部発表Yes

Fingerprint

Horseshoe Crabs
Hemocytes
Horseshoe
Cloning
Molecular Cloning
Nucleotides
CDNA
Messenger RNA
Amino acids
Amino Acids
Peptides
Proteins
Coding
Protein
Complementary DNA
Substitution reactions
Clone
Replacement
Substitution
Fragment

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

これを引用

Two types of coagulogen mRNAs found in horseshoe crab (Tachypleus tridentatus) hemocytes : Molecular cloning and nucleotide sequences. / Miyata, Toshiyuki; Matsumoto, Hiroaki; Hattori, Masahira; Sakaki, Yoshiyuki; Iwanaga, Sadaaki.

:: Journal of Biochemistry, 巻 100, 番号 1, 07.1986, p. 213-220.

研究成果: Article

Miyata, Toshiyuki ; Matsumoto, Hiroaki ; Hattori, Masahira ; Sakaki, Yoshiyuki ; Iwanaga, Sadaaki. / Two types of coagulogen mRNAs found in horseshoe crab (Tachypleus tridentatus) hemocytes : Molecular cloning and nucleotide sequences. :: Journal of Biochemistry. 1986 ; 巻 100, 番号 1. pp. 213-220.
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