抄録
The complete cDNA sequence coding for the coagulogen present in the horseshoe crab (Tachypleus tridentatus) hemocytes was determined. Clones carrying cDNA fragments for coagulogen were isolated from a cDNA library of the hemocyte mRNA using synthetic oligodeoxyribonuclcotides as probes. The nucleotide sequence analyses of the cloned cDNAs revealed that the hemocyte coagulogen consists of 175 amino acids with 20 amino acids in a presegment, and that there are two types of mRNAs for coagulogen. The two mRNAs exhibited three nucleotide substitutions, two of which were in their protein-coding regions, resulting in two amino acid replacements. Subsequently, two molecular species of coagulogen, named coagulogens type I and type II, were identified by tryptic peptide mapping of the mature proteins isolated from the hemocyte lysate. These results suggest that the two types of coagulogens are first synthesized as preproteins and are incorporated into the granules that are abundantly present in the hemocytes with liberation of the signal peptides.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 213-220 |
| ページ数 | 8 |
| ジャーナル | Journal of Biochemistry |
| 巻 | 100 |
| 発行部数 | 1 |
| 出版物ステータス | Published - 1986 7 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Molecular Biology
- Biochemistry
これを引用
Two types of coagulogen mRNAs found in horseshoe crab (Tachypleus tridentatus) hemocytes : Molecular cloning and nucleotide sequences. / Miyata, Toshiyuki; Matsumoto, Hiroaki; Hattori, Masahira; Sakaki, Yoshiyuki; Iwanaga, Sadaaki.
：: Journal of Biochemistry, 巻 100, 番号 1, 07.1986, p. 213-220.研究成果: Article
}
TY - JOUR
T1 - Two types of coagulogen mRNAs found in horseshoe crab (Tachypleus tridentatus) hemocytes
T2 - Molecular cloning and nucleotide sequences
AU - Miyata, Toshiyuki
AU - Matsumoto, Hiroaki
AU - Hattori, Masahira
AU - Sakaki, Yoshiyuki
AU - Iwanaga, Sadaaki
PY - 1986/7
Y1 - 1986/7
N2 - The complete cDNA sequence coding for the coagulogen present in the horseshoe crab (Tachypleus tridentatus) hemocytes was determined. Clones carrying cDNA fragments for coagulogen were isolated from a cDNA library of the hemocyte mRNA using synthetic oligodeoxyribonuclcotides as probes. The nucleotide sequence analyses of the cloned cDNAs revealed that the hemocyte coagulogen consists of 175 amino acids with 20 amino acids in a presegment, and that there are two types of mRNAs for coagulogen. The two mRNAs exhibited three nucleotide substitutions, two of which were in their protein-coding regions, resulting in two amino acid replacements. Subsequently, two molecular species of coagulogen, named coagulogens type I and type II, were identified by tryptic peptide mapping of the mature proteins isolated from the hemocyte lysate. These results suggest that the two types of coagulogens are first synthesized as preproteins and are incorporated into the granules that are abundantly present in the hemocytes with liberation of the signal peptides.
AB - The complete cDNA sequence coding for the coagulogen present in the horseshoe crab (Tachypleus tridentatus) hemocytes was determined. Clones carrying cDNA fragments for coagulogen were isolated from a cDNA library of the hemocyte mRNA using synthetic oligodeoxyribonuclcotides as probes. The nucleotide sequence analyses of the cloned cDNAs revealed that the hemocyte coagulogen consists of 175 amino acids with 20 amino acids in a presegment, and that there are two types of mRNAs for coagulogen. The two mRNAs exhibited three nucleotide substitutions, two of which were in their protein-coding regions, resulting in two amino acid replacements. Subsequently, two molecular species of coagulogen, named coagulogens type I and type II, were identified by tryptic peptide mapping of the mature proteins isolated from the hemocyte lysate. These results suggest that the two types of coagulogens are first synthesized as preproteins and are incorporated into the granules that are abundantly present in the hemocytes with liberation of the signal peptides.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022747274&partnerID=8YFLogxK
M3 - Article
C2 - 3759932
AN - SCOPUS:0022747274
VL - 100
SP - 213
EP - 220
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 1
ER -