Typing of X Chromosomes Bearing Tabby Allele in Mouse Preimplantation Embryos by Detection of a Microsatellite Marker

Ruby P A Valdivia, Tetsuo Kunieda, Masahira Hattori, Yutaka Toyoda

研究成果: Article

2 引用 (Scopus)

抜粋

Tabby (Ta) is a semidominat allele of the locus on the mouse X chromosome, which causes a characteristic coat pattern and developmental defects in endocrinic glands. To establish a method for identifying the X chromosome bearing the Ta allele in early preimplantation embryos, we performed PCR amplification of an X chromosomal microsatellite marker locus from preimplantation embryos obtained from mating between XO female and XY male mice. The microsatellite marker locus was shown to be polymorphic between X chromosomes bearing the Ta and wild-type alleles. The amplification of the marker locus from early preimplantation embryos demonstrated that the Ta locus can be correctly typed by this method from embryos as little as 2 cells.

元の言語English
ページ(範囲)395-398
ページ数4
ジャーナルExperimental Animals
45
発行部数4
出版物ステータスPublished - 1996 10
外部発表Yes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

フィンガープリント Typing of X Chromosomes Bearing Tabby Allele in Mouse Preimplantation Embryos by Detection of a Microsatellite Marker' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用