Ultra-fast Chara myosin: A test case for the swinging lever arm model for force production by myosin

Taro Q.P. Uyeda*

*この研究の対応する著者

研究成果: Short survey査読

7 被引用数 (Scopus)

抄録

Recent breakthroughs and technological improvements are rapidly generating evidence supporting the "swinging lever arm model" for force production by myosin. Unlike previous models, this model posits that the globular domain of the myosin motor binds to actin with a constant orientation during force generation. Movement of the neck domain of the motor is hypothesized to occur relative to the globular domain much like a lever arm. This intramolecular conformational change drives the movement of the bound actin. The swinging lever arm model is supported by or consistent with a large number of experimental data obtained with skeletal muscle or slime mold myosins, all of which move actin filaments at rates between 1 and 10 μm/s in vitro. Recently myosin was purified from Chara intemodal cells. In vitro, the purified Chara myosin moves actin filaments at rates one order of magnitude faster than the "fast" skeletal muscle myosin. While this ultra fast movement is not necessarily inconsistent with the swinging lever arm model, one or more specific facets of the motor must be altered in the Chara motor in order to accommodate such rapid movement. These characteristics are experimentally testable, thus the ultra fast movement by Chara myosin represents a powerful and compelling test of the swinging lever arm model.

本文言語English
ページ(範囲)231-239
ページ数9
ジャーナルJournal of Plant Research
109
3
DOI
出版ステータスPublished - 1996 9
外部発表はい

ASJC Scopus subject areas

  • 植物科学

フィンガープリント

「Ultra-fast Chara myosin: A test case for the swinging lever arm model for force production by myosin」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル