Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Yusuke Yoshioka, Nobuyoshi Kosaka, Yuki Konishi, Hideki Ohta, Hiroyuki Okamoto, Hikaru Sonoda, Ryoji Nonaka, Hirofumi Yamamoto, Hideshi Ishii, Masaki Mori, Koh Furuta, Takeshi Nakajima, Hiroshi Hayashi, Hajime Sugisaki, Hiroko Higashimoto, Takashi Kato, Fumitaka Takeshita, Takahiro Ochiya

研究成果: Article査読

256 被引用数 (Scopus)

抄録

Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

本文言語English
論文番号3591
ページ(範囲)3591
ページ数1
ジャーナルNature communications
5
DOI
出版ステータスPublished - 2014
外部発表はい

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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