Ultrasensitive detection of proteins and sugars at single-cell level

Satoshi Watabe, Mika Morikawa, Mugiho Kaneda, Kazunari Nakaishi, Akira Nakatsuma, Masaki Ninomiya, Teruki Yoshimura, Toshiaki Miura, Etsuro Ito*

*この研究の対応する著者

研究成果: Review article査読

13 被引用数 (Scopus)

抄録

Each cell produces its own responses even if it appears identical to other cells. To analyze these individual cell characteristics, we need to measure trace amounts of molecules in a single cell. Nucleic acids in a single cell can be easily amplified by polymerase chain reaction, but single-cell measurement of proteins and sugars will require de novo techniques. In the present study, we outline the techniques we have developed toward this end. For proteins, our ultrasensitive enzymelinked immunosorbent assay (ELISA) coupled with thionicotinamide-adenine dinucleotide cycling can detect proteins at subattomoles per assay. For sugars, fluorescence correlation spectroscopy coupled with glucose oxidase-catalyzed reaction allows us to measure glucose at tens of nM. Our methods thus offer versatile techniques for single-cell-level analyses, and they are hoped to strongly promote single-cell biology as well as to develop noninvasive tests in clinical medicine.

本文言語English
ジャーナルCommunicative and Integrative Biology
9
1
DOI
出版ステータスPublished - 2016
外部発表はい

ASJC Scopus subject areas

  • 農業および生物科学(全般)

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