抄録
The compartmentalization of a cell-free gene expression system inside a self-assembled lipid vesicle is envisioned as the simplest chassis for the construction of a minimal cell. Although crucial for its realization, quantitative understanding of the dynamics of gene expression in bulk and liposome-confined reactions is scarce. Here, we used two orthogonal fluorescence labeling tools to report the amounts of mRNA and protein produced in a reconstituted biosynthesis system, simultaneously and in real-time. The Spinach RNA aptamer and its fluorogenic probe were used for mRNA detection. Applying this dual-reporter assay to the analysis of transcript and protein production inside lipid vesicles revealed that their levels are uncorrelated, most probably a consequence of the low copy-number of some components in liposome-confined reactions. We believe that the stochastic nature of gene expression should be appreciated as a design principle for the assembly of a minimal cell.
本文言語 | English |
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ページ(範囲) | 1963-1966 |
ページ数 | 4 |
ジャーナル | ChemBioChem |
巻 | 14 |
号 | 15 |
DOI | |
出版ステータス | Published - 2013 10月 |
外部発表 | はい |
ASJC Scopus subject areas
- 生化学
- 分子医療
- 分子生物学
- 有機化学