TY - JOUR
T1 - UncI protein can mediate ring-assembly of c-subunits of FoF1-ATP synthase in vitro
AU - Ozaki, Yoko
AU - Suzuki, Toshiharu
AU - Kuruma, Yutetsu
AU - Ueda, Takuya
AU - Yoshida, Masasuke
PY - 2008/3/14
Y1 - 2008/3/14
N2 - In FoF1-ATP synthase, multimeric c-subunits are assembled to a ring (c-ring) in the membranes that rotates as protons flow across Fo. We recently reported that assembly of c-ring of Propionigenium modestum in the membranes of Escherichia coli cells required P. modestum UncI, a product of the conserved uncI gene in the FoF1 operon. However, cooperation with endogenous factors in E. coli remained unclear. Here, P. modestum c-subunit was synthesized in vitro in the presence of liposomes. When c-subunit alone was synthesized, it did not form c-ring. However, when c-subunit and P. modestum UncI were synthesized together, c-ring was formed. Fusion of the two kinds of liposomes, one containing only unassembled c-subunit and the other only UncI, resulted in gradual formation of c-ring. Thus, UncI alone can mediate in vitro post-translational c-ring assembly.
AB - In FoF1-ATP synthase, multimeric c-subunits are assembled to a ring (c-ring) in the membranes that rotates as protons flow across Fo. We recently reported that assembly of c-ring of Propionigenium modestum in the membranes of Escherichia coli cells required P. modestum UncI, a product of the conserved uncI gene in the FoF1 operon. However, cooperation with endogenous factors in E. coli remained unclear. Here, P. modestum c-subunit was synthesized in vitro in the presence of liposomes. When c-subunit alone was synthesized, it did not form c-ring. However, when c-subunit and P. modestum UncI were synthesized together, c-ring was formed. Fusion of the two kinds of liposomes, one containing only unassembled c-subunit and the other only UncI, resulted in gradual formation of c-ring. Thus, UncI alone can mediate in vitro post-translational c-ring assembly.
KW - ATP synthase
KW - Biogenesis of ATP synthase
KW - Chaperone
KW - In vitro protein synthesis
KW - Membrane integration
KW - Membrane proteins
KW - Motor enzyme
KW - UncI
KW - YidC
KW - c-Subunit
UR - http://www.scopus.com/inward/record.url?scp=38649086079&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38649086079&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.12.170
DO - 10.1016/j.bbrc.2007.12.170
M3 - Article
C2 - 18182163
AN - SCOPUS:38649086079
VL - 367
SP - 663
EP - 666
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -