Uni-directional growth of F-actin

Hiroshi Kondo*, Shin'ichi Ishiwata

*この研究の対応する著者

研究成果: Article査読

97 被引用数 (Scopus)

抄録

It was shown that the growth of F-actin occurs uni-directionally in vitro; that is, actin monomers (G. ADP-actin) polymerize onto only one of the two ends of a preexisting seed in the absence of ATP. Fragments of the F-actin-heavy meromyosin complex prepared by sonication were used as seeds. By studying a large number of electron micrographs, we concluded that growth occurs on the tail end of the arrowhead structure of the seed. This result suggests that the thin filament grows in vivo from the center of the sarcomere towards the Z-line. It was further shown that the fragments of F-actin combine with both ends of the seed with nearly equal probability. These results are well interpreted according to the theory deveoloped by Oosawa(1,2).

本文言語English
ページ(範囲)159-171
ページ数13
ジャーナルJournal of Biochemistry
79
1
出版ステータスPublished - 1976 1
外部発表はい

ASJC Scopus subject areas

  • 統計学、確率および不確実性
  • 応用数学
  • 生理学(医学)
  • 放射線学、核医学およびイメージング
  • 分子生物学
  • 生化学

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