Unidirectional cooperative binding of fimbrin actin-binding domain 2 to actin filament

Naoki Hosokawa, Masahiro Kuragano, Atsuki Yoshino, Keitaro Shibata, Taro Q.P. Uyeda, Kiyotaka Tokuraku*

*この研究の対応する著者

研究成果査読

1 被引用数 (Scopus)

抄録

Fimbrin forms bundles of parallel actin filaments in filopodia, but it remains unclear how fimbrin forms well-ordered bundles. To address this issue, we focused on the cooperative interaction between the actin-binding domain of fimbrin and actin filaments. First, we loosely immobilized actin filaments on a glass surface via a positively charged lipid layer and observed the binding of GFP-fused actin-binding domain 2 of fimbrin using fluorescence microscopy. The actin-binding domain formed low-density clusters with unidirectional growth along actin filaments. When the actin filaments were tightly immobilized to the surface by increasing the charge density of the lipid layer, cluster formation was suppressed. This result suggests that the propagation of cooperative structural changes of actin filaments evoked by binding of the actin-binding domain was suppressed by a strong physical interaction with the glass surface. Interestingly, binding of the fimbrin actin-binding domain shortened the length of loosely immobilized actin filaments. Based on these results, we propose that fimbrin-actin interactions accompanied by unidirectional long-range allostery help the formation of well-ordered parallel actin filament bundles.

本文言語English
ページ(範囲)59-65
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
552
DOI
出版ステータスPublished - 2021 5 7

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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