Uniform and high throughput agarose gel micro droplet generation device for single cell analysis

T. Hirose, Y. Hoshino, Donghyun Yoon, A. Nakahara, T. Mori, Tetsushi Sekiguchi, Haruko Takeyama, Shuichi Shoji

研究成果: Conference contribution

抄録

We designed and fabricated high throughput agarose gel micro droplet (GMD) generation device for single cell function analysis. Temperature control of each elements of the device is important issue because of the thermo-oriented sol-gel transfer of agarose gel. Uniform GMD of 50 μm in diameter which is suitable size for cell culturing media is achieved with size deviation less than 1.58% and generation rate of about 190deoplets/sec. Single E.coli cell encapsulation and cultivation for 5 hours are successfully achieved in an agarose GMD.

元の言語English
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版者Chemical and Biological Microsystems Society
ページ500-502
ページ数3
ISBN(印刷物)9780979806452
出版物ステータスPublished - 2012
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa
継続期間: 2012 10 282012 11 1

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Okinawa
期間12/10/2812/11/1

Fingerprint

Sepharose
Gels
Throughput
Encapsulation
Temperature control
Escherichia coli
Sol-gels

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

これを引用

Hirose, T., Hoshino, Y., Yoon, D., Nakahara, A., Mori, T., Sekiguchi, T., ... Shoji, S. (2012). Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. : Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 500-502). Chemical and Biological Microsystems Society.

Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. / Hirose, T.; Hoshino, Y.; Yoon, Donghyun; Nakahara, A.; Mori, T.; Sekiguchi, Tetsushi; Takeyama, Haruko; Shoji, Shuichi.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 500-502.

研究成果: Conference contribution

Hirose, T, Hoshino, Y, Yoon, D, Nakahara, A, Mori, T, Sekiguchi, T, Takeyama, H & Shoji, S 2012, Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. : Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 500-502, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, 12/10/28.
Hirose T, Hoshino Y, Yoon D, Nakahara A, Mori T, Sekiguchi T その他. Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. : Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 500-502
Hirose, T. ; Hoshino, Y. ; Yoon, Donghyun ; Nakahara, A. ; Mori, T. ; Sekiguchi, Tetsushi ; Takeyama, Haruko ; Shoji, Shuichi. / Uniform and high throughput agarose gel micro droplet generation device for single cell analysis. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 500-502
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AU - Sekiguchi, Tetsushi

AU - Takeyama, Haruko

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