Unusual priming mechanism of RNA-directed DNA synthesis in copia retrovirus-like particles of Drosophila

Yo Kikuchi*, Yumiko Ando, Tadayoshi Shiba

*この研究の対応する著者

研究成果: Article査読

61 被引用数 (Scopus)

抄録

Drosophila cells contain virus-like particles (VLPs) containing 5 kilobases (kb) of RNA (VLP H-RNA) homologous to the transposable element copia 1. The identity between VLP H-RNA and copia DNA has previously been confirmed at the nucleotide sequence level2 and reverse transcriptase activity is also detected in the VLPs1. These results suggest that VLPs and copia are derivatives of viral particle and provirus forms, respectively, of the copia retrovirus-like particle. If the copia retrovirus-like particle replicates by a mechanism similar to the mechanism of vertebrate retroviral replication, a cellular transfer RNA would prime synthesis of the first DNA strand. We show that this is indeed so but that copia retrovirus-like particle has a novel type of priming mechanism; the first DNA extension does not start from the 3′ end of a tRNA, but from an internal site (two nucleotides after the anticodon loop) of the Drosophila initiator methionine tRNA (tRNAi Met)

本文言語English
ページ(範囲)824-826
ページ数3
ジャーナルNature
323
6091
DOI
出版ステータスPublished - 1986
外部発表はい

ASJC Scopus subject areas

  • 一般

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