Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion

Shinichi Komaba, Michiko Seyama, Katsuhisa Tanabe, Tetsuya Osaka

研究成果: Article

7 引用 (Scopus)

抄録

A urea sensor based on the composite film of electroinactive polypyrrole (PPy) and urease (Urs) modified with polyanion was fabricated by electropolymerization. This sensor showed the potential response to urea based on the detection of pH-change during the enzymatic reaction by Urs. The sensitivity of this sensor was 53 mV decade-1 which was higher than that of the PPy film coated electrode in which Urs was entrapped physically as previously reported. The high sensitivity was considered to be attributed to the increase in the number of immobilized Urs with the covalent bonding with carboxyl groups of PAA. This urea sensor showed linear potential response between 1 × 10-4 mol dm-3 and 1 × 10-1 mol dm-3.

元の言語English
ページ(範囲)1228-1233
ページ数6
ジャーナルDenki Kagaku
64
発行部数12
出版物ステータスPublished - 1996

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Urease
Polypyrroles
Composite films
Biosensors
Urea
Sensors
Electropolymerization
Electrodes
polypyrrole
polyanions

ASJC Scopus subject areas

  • Chemistry(all)

これを引用

Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion. / Komaba, Shinichi; Seyama, Michiko; Tanabe, Katsuhisa; Osaka, Tetsuya.

:: Denki Kagaku, 巻 64, 番号 12, 1996, p. 1228-1233.

研究成果: Article

Komaba, Shinichi ; Seyama, Michiko ; Tanabe, Katsuhisa ; Osaka, Tetsuya. / Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion. :: Denki Kagaku. 1996 ; 巻 64, 番号 12. pp. 1228-1233.
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