Urinary excretion of cytokines versus their plasma levels after endurance exercise

Kaoru Sugama, Katsuhiko Suzuki, Kayo Yoshitani, Koso Shiraishi, Takashi Kometani

研究成果: Article

21 引用 (Scopus)

抄録

It has been consistently shown that circulating levels of interleukin (IL)-6, IL-8, IL-1 receptor antagonist (IL-1ra) and IL-10 increase remarkably following endurance exercise longer than 2 h such as marathon and triathlon races. However, no studies have compared changes in the plasma and urinary levels of these cytokines after endurance exercise, including the recovery period. In the present study, we investigated kinetic changes in the urinary excretion of cytokines following endurance exercise up to 3 h after exercise to evaluate the magnitude of change in comparison to the plasma levels and to explore the possible biological significance and the mechanisms of cytokine dynamics following exercise. Fourteen male athletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling, and 5 km of running. Venous blood and urine samples were collected before, immediately after, 1.5 h and 3 h after the race. Plasma and urine were analyzed using enzyme-linked immunosorbent assays (ELISA). Plasma concentrations of IL-1β, IL-1ra, IL-6, IL-8, IL-10 and monocyte chemotactic protein (MCP)-1 increased significantly after the race, whereas tumour necrosis factor (TNF)-α, IL-2, IL-4, IL-12 and interferon (IFN)-γ did not change significantly. Urinary concentrations of IL-1β, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFN- γ and MCP-1 increased significantly after the race. When the urine concentrations were adjusted by creatinine concentration, urine volume and sampling time, the increases of IL-2, IL-4, IL-8, IL-10, IFN-γ and MCP-1 were evident and these were notably present in urine of the stressed athletes suffering from renal tubular epithelial damage. The present study provides new evidence that the kinetics and magnitude of changes in urinary cytokine concentrations differ from plasma cytokine concentrations following endurance exercise, especially, in the recovery period several hours after exercise, and that the damaged kidney might be responsible at least in part for the kinetics of some cytokines. Urinary cytokines may be sensitive biomarkers of the impact of exhaustive exercise workload on renal damage and inflammation in the recovery period after endurance exercise.

元の言語English
ページ(範囲)29-48
ページ数20
ジャーナルExercise Immunology Review
19
出版物ステータスPublished - 2013

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Exercise
Cytokines
Interleukin-8
Interleukin-10
Urine
Chemokine CCL2
Interleukin-4
Interferons
Interleukin-2
Interleukin-6
Interleukins
Interleukin-12
Interleukin-1
Kidney
Running
Athletes
Interleukin-1 Receptors
Workload
Creatinine
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Immunology

これを引用

Sugama, K., Suzuki, K., Yoshitani, K., Shiraishi, K., & Kometani, T. (2013). Urinary excretion of cytokines versus their plasma levels after endurance exercise. Exercise Immunology Review, 19, 29-48.

Urinary excretion of cytokines versus their plasma levels after endurance exercise. / Sugama, Kaoru; Suzuki, Katsuhiko; Yoshitani, Kayo; Shiraishi, Koso; Kometani, Takashi.

:: Exercise Immunology Review, 巻 19, 2013, p. 29-48.

研究成果: Article

Sugama, K, Suzuki, K, Yoshitani, K, Shiraishi, K & Kometani, T 2013, 'Urinary excretion of cytokines versus their plasma levels after endurance exercise', Exercise Immunology Review, 巻. 19, pp. 29-48.
Sugama, Kaoru ; Suzuki, Katsuhiko ; Yoshitani, Kayo ; Shiraishi, Koso ; Kometani, Takashi. / Urinary excretion of cytokines versus their plasma levels after endurance exercise. :: Exercise Immunology Review. 2013 ; 巻 19. pp. 29-48.
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