Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

Hiroyasu Itoh; Ariella Evenzahav; Katsuyuki Kinoshita; Yoshinori Inagaki; Hiroshi Mizushima; Akira Takahashi; Tsuyoshi Hayakawa; Kazuhiko Kinosita

doi:10.1117/12.280219

Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

Hiroyasu Itoh^*, Ariella Evenzahav, Katsuyuki Kinoshita, Yoshinori Inagaki, Hiroshi Mizushima, Akira Takahashi, Tsuyoshi Hayakawa, Kazuhiko Kinosita

^*この研究の対応する著者

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

本文言語	English
ホスト出版物のタイトル	Proceedings of SPIE - The International Society for Optical Engineering
編集者	B. Chance, R.R. Alfano, A. Katzir
ページ	733-740
ページ数	8
巻	2979
DOI	https://doi.org/10.1117/12.280219
出版ステータス	Published - 1997
外部発表	はい
イベント	Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II - San Jose, CA, United States 継続期間: 1997 2月 9 → 1997 2月 12

Other

Other	Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II
国/地域	United States
City	San Jose, CA
Period	97/2/9 → 97/2/12

ASJC Scopus subject areas

電子工学および電気工学
凝縮系物理学

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10.1117/12.280219

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Itoh, H., Evenzahav, A., Kinoshita, K., Inagaki, Y., Mizushima, H., Takahashi, A., Hayakawa, T., & Kinosita, K. (1997). Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena. In B. Chance, R. R. Alfano, & A. Katzir (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2979, pp. 733-740) https://doi.org/10.1117/12.280219

Itoh, H, Evenzahav, A, Kinoshita, K, Inagaki, Y, Mizushima, H, Takahashi, A, Hayakawa, T & Kinosita, K 1997, Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena. in B Chance, RR Alfano & A Katzir (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 2979, pp. 733-740, Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II, San Jose, CA, United States, 97/2/9. https://doi.org/10.1117/12.280219

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title = "Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena",

abstract = "A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.",

keywords = "Cellular phenomena, Dual-view assembly, Fluorescence lifetime imaging microscopy (FLIM), Framing camera, Frequency domain, Phase-resolved",

author = "Hiroyasu Itoh and Ariella Evenzahav and Katsuyuki Kinoshita and Yoshinori Inagaki and Hiroshi Mizushima and Akira Takahashi and Tsuyoshi Hayakawa and Kazuhiko Kinosita",

year = "1997",

doi = "10.1117/12.280219",

language = "English",

volume = "2979",

pages = "733--740",

editor = "B. Chance and R.R. Alfano and A. Katzir",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

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AU - Itoh, Hiroyasu

AU - Evenzahav, Ariella

AU - Kinoshita, Katsuyuki

AU - Inagaki, Yoshinori

AU - Mizushima, Hiroshi

AU - Takahashi, Akira

AU - Hayakawa, Tsuyoshi

AU - Kinosita, Kazuhiko

PY - 1997

Y1 - 1997

N2 - A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

AB - A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

KW - Cellular phenomena

KW - Dual-view assembly

KW - Fluorescence lifetime imaging microscopy (FLIM)

KW - Framing camera

KW - Frequency domain

KW - Phase-resolved

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DO - 10.1117/12.280219

M3 - Conference contribution

AN - SCOPUS:0031288719

VL - 2979

SP - 733

EP - 740

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Chance, B.

A2 - Alfano, R.R.

A2 - Katzir, A.

T2 - Proceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II

Y2 - 9 February 1997 through 12 February 1997

ER -

Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

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