Use of a gain modulating framing camera for time-resolved imaging of cellular phenomena

Hiroyasu Itoh*, Ariella Evenzahav, Katsuyuki Kinoshita, Yoshinori Inagaki, Hiroshi Mizushima, Akira Takahashi, Tsuyoshi Hayakawa, Kazuhiko Kinosita

*この研究の対応する著者

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

A gain modulating framing camera and its application towards the study of real time cellular phenomena will be described. Based on a unique operating principle, this framing camera can be modulated by over 90% at 1 GHz. The camera consists of an image converter with a pair of deflection electrodes and a rectangular aperture. Since a sinusoidal electric field is applied to the deflection electrodes, the photoelectron image-forming beam is continuously deflected and swept on the aperture. A bias is applied to center the sweep of the photoelectron beam on the edge of the aperture. The gain modulating with high depth can hence be accomplished. We are now constructing a fluorescence lifetime imaging microscope system employing this gain modulating framing camera based on the phase domain method. Such high depth modulation enables us to achieve frequency signals as low as 1 Hz in heterodyne operation. We will describe examples of application of the system towards the observation of various cellular phenomena.

本文言語English
ホスト出版物のタイトルProceedings of SPIE - The International Society for Optical Engineering
編集者B. Chance, R.R. Alfano, A. Katzir
ページ733-740
ページ数8
2979
DOI
出版ステータスPublished - 1997
外部発表はい
イベントProceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II - San Jose, CA, United States
継続期間: 1997 2 91997 2 12

Other

OtherProceedings of Optical Tomography and Spectroscopy of Tissue: Theory, Instrumentation, Model and Human Studies II
国/地域United States
CitySan Jose, CA
Period97/2/997/2/12

ASJC Scopus subject areas

  • 電子工学および電気工学
  • 凝縮系物理学

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