TY - JOUR
T1 - Use of anion exchange resin-packed capillary column for rapid detection of anti-double-stranded DNA antibody in systemic lupus erythematosus serum
AU - Lim, Tae Kyu
AU - Nakamura, Noriyuki
AU - Matsunaga, Tadashi
PY - 2000/6/5
Y1 - 2000/6/5
N2 - An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between luminescence intensity and concentration of anti-dsDNA antibody in the range 25-200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns. (C) 2000 John Wiley and Sons, Inc.
AB - An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between luminescence intensity and concentration of anti-dsDNA antibody in the range 25-200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns. (C) 2000 John Wiley and Sons, Inc.
KW - Alkaline phosphatase conjugated DNA
KW - Anion exchange capillary column
KW - Anti-dsDNA antibody
KW - Isoelectric point
KW - Systemic lupus erythematosus
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U2 - 10.1002/(SICI)1097-0290(20000605)68:5<571::AID-BIT12>3.0.CO;2-D
DO - 10.1002/(SICI)1097-0290(20000605)68:5<571::AID-BIT12>3.0.CO;2-D
M3 - Article
C2 - 10797244
AN - SCOPUS:0034608597
VL - 68
SP - 571
EP - 575
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
SN - 0006-3592
IS - 5
ER -