Use of anion exchange resin-packed capillary column for rapid detection of anti-double-stranded DNA antibody in systemic lupus erythematosus serum

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

5 被引用数 (Scopus)

抄録

An anti-double-stranded (ds) DNA antibodies in serum-detection system was developed based on flow immunoassay with packed-capillary column. Alkaline phosphatase conjugated DNA (ALP-DNA) and anti-dsDNA antibody were separated from unreacted ALP-DNA on the basis of the difference in isoelectric point. A linear anti-dsDNA antibody dose-response curve was obtained between luminescence intensity and concentration of anti-dsDNA antibody in the range 25-200 IU/mL. This simple technique permits the assay of anti-dsDNA autoimmune antibodies within 7 minutes. These results give reduced detection times compared with those previously reported (Lim et al., 1999) through the use of capillary columns. (C) 2000 John Wiley and Sons, Inc.

本文言語English
ページ(範囲)571-575
ページ数5
ジャーナルBiotechnology and Bioengineering
68
5
DOI
出版ステータスPublished - 2000 6 5
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 微生物学

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