Use of real-time PCR to examine the relationship between ammonia oxidizing bacterial populations and nitrogen removal efficiency in a small decentralized treatment system 'Johkasou'

G. Nakagawa, Y. Ebie, S. Tsuneda, M. Matsumura, Y. Inamori

研究成果: Conference contribution

10 被引用数 (Scopus)

抄録

The aim of this study was to examine the relationship between ammonia oxidizing bacterial populations and biological nitrogen removal in a small on-site domestic wastewater treatment system "Johkasou". The population dynamics of ammonia oxidizing bacteria (AOB) in six full-scale advanced Johkasous was surveyed using real-time PCR assay over a period of one year. These Johkasous were selected to compare the AOB populations in different treatment performance. When the effluent NH4-N concentration was higher than 2 mg L-1, it was difficult to meet the effluent standard of advanced Johkasous (T-N 10 mg L-1). In contrast, the nitrogen removal efficiency was hardly affected by nitrite oxidation and denitrification in these systems. In other words, ammonia oxidation was a rate-limiting step. Furthermore, we focused on the relationship between NH4-N loading per AOB cell and nitrogen removal. Real time PCR monitoring results demonstrated that it is important to regulate NH4-N loading per AOB cell below 210 pg cell-1 day-1 to meet the effluent standard of advanced Johkasou. It is considered that NH4-N loading per AOB cell is a useful parameter for determining suitable nitrogen loading and small decentralized system design.

本文言語English
ホスト出版物のタイトルSmall Water and Wastewater Systems VII
編集者S.G. Martinez
ページ203-210
ページ数8
7
DOI
出版ステータスPublished - 2007 5 22

出版物シリーズ

名前Water Science and Technology
番号7
55
ISSN(印刷版)0273-1223

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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