Use of silk fibroin for enzyme membrane

Akio Kuzuhara, Tetsuo Asakura, Ryuzo Tomoda, Tadashi Matsunaga

研究成果: Article査読

83 被引用数 (Scopus)

抄録

Bombyx mori and Philosamia cynthia ricini silk fibroins were employed for preparing enzyme membranes. Glucose oxidase (GOD) was entrapped in the silk fibroin membrane without glutaraldehyde treatment. The entrapped GOD was tested with the colorimetric method. The activity yield of GOD was 98.7% when 3.71 × 10-3 U of GOD were immobilized in 1.1 mg dry weight B. mori silk fibroin membrane (300 μm). The enzyme leakage from the enzyme membrane was only 0.05% after 36 days. In addition, the activity after heating at 60°C for 20 min was unchanged. IR spectra indicated that the GOD-B. mori silk fibroin membrane contained 80% random coil and 20% silk II (anti-parallel β-sheet) structures. When it was soaked in 80% methanol for 30 s, the ratio of silk II changed to 50%. These results explained that the densely packed surface prevented the GOD from leaking.

本文言語English
ページ(範囲)199-207
ページ数9
ジャーナルJournal of Biotechnology
5
3
DOI
出版ステータスPublished - 1987
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

フィンガープリント 「Use of silk fibroin for enzyme membrane」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル