Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size

Jun Takagi, Takeshi Itabashi, Kazuya Suzuki, Tarun M. Kapoor, Yuta Shimamoto, Shin'ichi Ishiwata*

*この研究の対応する著者

    研究成果: Article査読

    5 被引用数 (Scopus)

    抄録

    The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured these parameters using 3D microscopy of meiotic spindles thatself-organized in Xenopus egg extracts and presented a simple equation describing the relationship among these parameters. To examine the validity of the equation, we cut the spindle into two fragments along the pole-to-pole axis by micromanipulation techniques that rapidly decrease the amount of MTs. The spheroidal shape spontaneously recovered within 5min, but the size of each fragment remained small. The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density

    本文言語English
    ページ(範囲)44-50
    ページ数7
    ジャーナルCell Reports
    5
    1
    DOI
    出版ステータスPublished - 2013 10 17

    ASJC Scopus subject areas

    • 生化学、遺伝学、分子生物学(全般)

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