Using the effective thermal conductivity to evaluate the freezing process in Medaka embryos suspension

Naoki Aizawa*, Nobusuke Shimamoto, Masanobu Ujihira, Kazuo Tanishita

*この研究の対応する著者

研究成果: Article査読

抄録

The feasibility of using thermophysical properties to evaluate extracellular freezing processes favorable for cryopreservation was demonstrated by determining the correlation between the thermal state and the viability of cells and tissue. We measured the effective thermal conductivity of Medaka embryos (Oryzias latipes) during extracellular freezing using the self-heated thermistor technique. The insulated test chamber (8x8x12 mm) had a 2.5-mm-diameter thermistor bead installed in the bottom. The effective thermal conductivity was measured at -10°C for various cooling rates (from 0.1 to 10°C/min) for embryos in 1.92 mol/kg DMSO, and measured from 6 to -20°C for a cooling rate of 1°C/min for embryos in 0 - 2.56 mol/kg DMSO. We used the hatching rate to evaluate the viability of the embryos. The effective thermal conductivity was strongly correlated with the hatching rate, increasing as the hatching rate decreased. This demonstrates that thermophysical properties of biological materials can be used as indices for evaluating the freezing process of such materials.

本文言語English
ページ(範囲)768-776
ページ数9
ジャーナルJSME International Journal, Series C: Mechanical Systems, Machine Elements and Manufacturing
42
3
出版ステータスPublished - 1999 9
外部発表はい

ASJC Scopus subject areas

  • 産業および生産工学
  • 機械工学

フィンガープリント

「Using the effective thermal conductivity to evaluate the freezing process in Medaka embryos suspension」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル