UV-mediated ligand immobilization for multiplexed analysis in extended nanospace channels

Bjorn Renberg, Kazuma Mawatari, Takehiko Tsukahara, Naokazu Idota, Kae Sato, Takehiko Kitamori

研究成果: Conference contribution

抄録

A general method for immobilization of capture ligands in a single channel of extended nanospace depth is reported. For multiplexed analysis in a single channel, several ligands must be immobilized in the same channel. Since the bonding procedure (by heat/ HF) for glass and fused silica chips would destroy the ligands, we designed and synthesized a UV-activated linker for covalent ligand attachment inside the channels after fabrication. A modified mask aligner was used to align OHP or Cr masks to the channels to facilitate immobilization of the DNA capture oligos to pre-defined areas.

本文言語English
ホスト出版物のタイトル12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
出版社Chemical and Biological Microsystems Society
ページ1528-1530
ページ数3
出版ステータスPublished - 2008
外部発表はい
イベント12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
継続期間: 2008 10 122008 10 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CitySan Diego, CA
Period08/10/1208/10/16

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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