Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

Maki Ohtani, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo*, Ikuo Hirono, Haruko Takeyama, Takashi Aoki

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.

本文言語English
ページ(範囲)724-728
ページ数5
ジャーナルFish and Shellfish Immunology
34
2
DOI
出版ステータスPublished - 2013 2

ASJC Scopus subject areas

  • 環境化学
  • 水圏科学

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