Visualization of distribution of endotoxin trapped in an endotoxin-blocking filtration membrane

Masayo Hayama, Takehiro Miyasaka, Seiichi Mochizuki, Hiroko Asahara, Katsuhiko Tsujioka, Fukashi Kohori, Kiyotaka Sakai, Yoichi Jinbo, Masashi Yoshida

    研究成果: Article

    16 引用 (Scopus)

    抄録

    Since the finding of β2-microglobulin as a causal substance in the carpal tunnel syndrome of chronic hemodialysis patients, removal of β2-microglobulin has been performed using highly permeable dialysis membranes with larger pores. Such large-pore membranes tend to allow endotoxins (Et), harmful substances contained in dialysate, to enter blood. At present, as a countermeasure, Et-blocking filtration membranes are used to remove Et from dialysate. However, Et removal mechanism by these membranes has not been clarified yet. The objective of this study is thus to visualize distribution of fluorescence-labeled Et trapped inside the polyester-polymer alloy (PEPA) membrane, a widely used Et-blocking filtration membrane using a confocal laser scanning fluorescence microscope (CLSFM). Et were observed mainly in the outer skin layer of the hollow fiber, while some in the void and inner skin layers. No Et were present inside the hollow fiber. In conclusion, we succeeded in visualization of Et distribution inside the Et-blocking filtration membrane using CLSFM. This novel visualization technique may allow evaluation of distribution of Et trapped inside various kinds of Et-blocking filtration membranes.

    元の言語English
    ページ(範囲)45-53
    ページ数9
    ジャーナルJournal of Membrane Science
    210
    発行部数1
    DOI
    出版物ステータスPublished - 2002 12 1

    Fingerprint

    endotoxins
    Endotoxins
    Visualization
    membranes
    Membranes
    Fluorescence
    Skin
    Microscopes
    Dialysis membranes
    Scanning
    Dialysis Solutions
    Fibers
    Lasers
    fluorescence
    hollow
    Polyesters
    microscopes
    Tunnels
    Blood
    porosity

    ASJC Scopus subject areas

    • Filtration and Separation
    • Polymers and Plastics

    これを引用

    Visualization of distribution of endotoxin trapped in an endotoxin-blocking filtration membrane. / Hayama, Masayo; Miyasaka, Takehiro; Mochizuki, Seiichi; Asahara, Hiroko; Tsujioka, Katsuhiko; Kohori, Fukashi; Sakai, Kiyotaka; Jinbo, Yoichi; Yoshida, Masashi.

    :: Journal of Membrane Science, 巻 210, 番号 1, 01.12.2002, p. 45-53.

    研究成果: Article

    Hayama, M, Miyasaka, T, Mochizuki, S, Asahara, H, Tsujioka, K, Kohori, F, Sakai, K, Jinbo, Y & Yoshida, M 2002, 'Visualization of distribution of endotoxin trapped in an endotoxin-blocking filtration membrane', Journal of Membrane Science, 巻. 210, 番号 1, pp. 45-53. https://doi.org/10.1016/S0376-7388(02)00373-3
    Hayama, Masayo ; Miyasaka, Takehiro ; Mochizuki, Seiichi ; Asahara, Hiroko ; Tsujioka, Katsuhiko ; Kohori, Fukashi ; Sakai, Kiyotaka ; Jinbo, Yoichi ; Yoshida, Masashi. / Visualization of distribution of endotoxin trapped in an endotoxin-blocking filtration membrane. :: Journal of Membrane Science. 2002 ; 巻 210, 番号 1. pp. 45-53.
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    abstract = "Since the finding of β2-microglobulin as a causal substance in the carpal tunnel syndrome of chronic hemodialysis patients, removal of β2-microglobulin has been performed using highly permeable dialysis membranes with larger pores. Such large-pore membranes tend to allow endotoxins (Et), harmful substances contained in dialysate, to enter blood. At present, as a countermeasure, Et-blocking filtration membranes are used to remove Et from dialysate. However, Et removal mechanism by these membranes has not been clarified yet. The objective of this study is thus to visualize distribution of fluorescence-labeled Et trapped inside the polyester-polymer alloy (PEPA) membrane, a widely used Et-blocking filtration membrane using a confocal laser scanning fluorescence microscope (CLSFM). Et were observed mainly in the outer skin layer of the hollow fiber, while some in the void and inner skin layers. No Et were present inside the hollow fiber. In conclusion, we succeeded in visualization of Et distribution inside the Et-blocking filtration membrane using CLSFM. This novel visualization technique may allow evaluation of distribution of Et trapped inside various kinds of Et-blocking filtration membranes.",
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    AU - Kohori, Fukashi

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