Vitamin C depletion increases superoxide generation in brains of SMP30/GNL knockout mice

Yoshitaka Kondo, Toru Sasaki, Yasunori Sato, Akiko Amano, Shingo Aizawa, Mizuki Iwama, Setsuko Handa, Nobuko Shimada, Mitsugu Fukuda, Masumi Akita, Jaewon Lee, Kyu Shik Jeong, Naoki Maruyama, Akihito Ishigami*


研究成果: Article査読

68 被引用数 (Scopus)


Vitamin C (VC) has a strong antioxidant function evident as its ability to scavenge superoxide radicals in vitro. We verified that this property actually exists in vivo by using a real-time imaging system in which Lucigenin is the chemiluminescent probe for detecting superoxide in senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo. SMP30/GNL KO mice were given 1.5 g/L VC [VC(+)] for 2, 4, or 8 weeks or denied VC [VC(-)]. At 4 and 8 weeks, VC levels in brains from VC(-) KO mice were <6% of that in VC(+) KO mice. Accordingly, superoxide-dependent chemiluminescence levels determined by ischemia-reperfusion at the 4- and 8 weeks test intervals were 3.0-fold and 2.1-fold higher, respectively, in VC(-) KO mice than in VC(+) KO mice. However, total superoxide dismutase activity and protein levels were not altered. Thus, VC depletion specifically increased superoxide generation in a model of the living brain.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 2008 12 5

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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