Vitamin C is not essential for carnitine biosynthesis in vivo: Verification in vitamin C-depleted senescence marker protein-30/gluconolactonase knockout mice

Hajime Furusawa, Yasunori Sato, Yasukazu Tanaka, Yoko Inai, Akiko Amano, Mizuki Iwama, Yoshitaka Kondo, Setsuko Handa, Akira Murata, Morimitsu Nishikimi, Sataro Goto, Naoki Maruyama, Ryoya Takahashi, Akihito Ishigami

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Carnitine is an essential cofactor in the transport of long-chain fatty acids into the mitochondrial matrix and plays an important role in energy production via β-oxidation. Vitamin C (VC) has long been considered a requirement for the activities of two enzymes in the carnitine biosynthetic pathway, i.e., 6-N-trimethyllysine dioxygenase and γ-butyrobetaine dioxygenase. Our present study using senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize VC in vivo, led to the conclusion that this notion is not true. After weaning at 40 d of age, SMP30/GNL KO mice were fed a diet lacking VC and carnitine, then given water containing 1.5 g/l VC (VC(+) mice) or no VC (VC(-) mice) for 75 d. Subsequently, total VC and carnitine levels were measured in the cerebrum, cerebellum, liver, kidney, soleus muscle, extensor digitorum longus muscle, heart, plasma and serum. The total VC levels in all tissues and plasma from VC(-) SMP30/GNL KO mice were negligible, i.e., <2% of the levels in SMP30/GNL KO VC(+) mice; however, the total carnitine levels of both groups were similar in all tissues and serum. In addition, carnitine was produced by incubated liver homogenates from the VC-depleted SMP30/GNL KO mice irrespective of the presence or absence of 1mM VC. Collectively, these results indicate that VC is not essential for carnitine biosynthesis in vivo.

本文言語English
ページ(範囲)1673-1679
ページ数7
ジャーナルBiological and Pharmaceutical Bulletin
31
9
DOI
出版ステータスPublished - 2008 9
外部発表はい

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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