Voltage-induced pore formation and hemolysis of human erythrocytes

Kazuhiko Kinosita, Tian Yow Tsong

研究成果: Article

261 引用 (Scopus)

抄録

Isotonic suspensions of human erythrocytes were exposed to single electric pulses of intensity at a few kV/cm and duration in microseconds. Upon pulsation, the cell membranes became permeable to Na+ and K+, and the erythrocytes eventually hemolysed through the colloid osmotic effect of hemoglobin. The enhanced permeability is attributed to the formation of pores in the cell membranes. These pores are formed within a fraction of a microsecond, once the transmembrane potential induced by the applied electric field reaches a critical value of 1.0 V. Increased field intensity and pulse duration, or pulsation at low ionic strengths all expand the pore size, leading to an accelerated hemolysis reaction. In contrast to this expansion process, the initial step of pore formation is governed solely by the magnitude of the transmembrane potential: the critical value of the potential stays essentially constant in media of different ionc strengths, nor does it change appreciably with varying pulse duration. An abrupt increase in membrane permeability at a transmembrane potential around 1 V has been observed in many cellular systems. It is suggested that a similar mechanism of pore formation may apply to these systems as well.

元の言語English
ページ(範囲)227-242
ページ数16
ジャーナルBBA - Biomembranes
471
発行部数2
DOI
出版物ステータスPublished - 1977 12 1
外部発表Yes

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Cell membranes
Hemolysis
Membrane Potentials
Erythrocytes
Permeability
Colloids
Electric potential
Ionic strength
Cell Membrane
Pore size
Suspensions
Hemoglobins
Electric fields
Membranes
Osmolar Concentration

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

これを引用

Voltage-induced pore formation and hemolysis of human erythrocytes. / Kinosita, Kazuhiko; Tsong, Tian Yow.

:: BBA - Biomembranes, 巻 471, 番号 2, 01.12.1977, p. 227-242.

研究成果: Article

Kinosita, Kazuhiko ; Tsong, Tian Yow. / Voltage-induced pore formation and hemolysis of human erythrocytes. :: BBA - Biomembranes. 1977 ; 巻 471, 番号 2. pp. 227-242.
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