Wall shear stress distribution inside induced cerebral aneurysm on rabbit

T. Tanoue, S. Tateshima, D. Wakui, F. Vinuela, R. Sudo, K. Tanishita

研究成果: Conference contribution

1 引用 (Scopus)

抄録

Introduction: Cerebral aneurysm is a cerebrovascular disease resulting to subarachnoid hemorrhage. The generation, development and finally rupture of cerebral aneurysm could be related to hemodynamic stimulate, especially wall shear stress (WSS). WSS have been considered to play a role on the expression of such enzymes as iNOS, MMP-2 and MMP-9 leading to weakening of the arterial wall. However, detailed WSS distribution contributing to the enzyme expression has not been clarified inside cerebral artery. The purpose of our study is to clarify the WSS distribution corresponding to the enzyme expressions. Materials and Methods: We tried to induce cerebral aneurysm on rabbits, and created the realistic silicone model of the induced aneurysm. The flow field and WSS were measured using PIV (particle image velocimetry) and LDV (laser Doppler velocimeter) inside the realistic model. Furthermore, histological structure and enzyme expression were evaluated on the image of elastica van Gieson stain and on the immunostaining images. Results: Induced cerebral aneurysms were found in posterior circulation; the arterial bifurcation at basilar artery or posterior communicating artery. The induced cerebral aneurysm had an arterial wall structure with thinned media and lacked IEL. The thinned media was observed at the bulge top area wth low WSS distribution. On the other hand, lacked IEL was observed near the aneurysm neck area. Although WSS was not so low at the area of lacked IEL, the WSS spatial gradient appeared with high value at this area. The lack of occurs before the thinning of media on the process of aneurysm formation. In addition, MMP-2 and MMP-9 showed similar trend as the WSS spatial gradient. From these findings, high WSS gradient seems to have an effect on the early stage of cerebral aneurysm generation, stimulating the enzymes expression which leads to the destruction of the IEL.

元の言語English
ホスト出版物のタイトルIFMBE Proceedings
ページ1063-1066
ページ数4
31 IFMBE
DOI
出版物ステータスPublished - 2010
外部発表Yes
イベント6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech - Singapore
継続期間: 2010 8 12010 8 6

Other

Other6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech
Singapore
期間10/8/110/8/6

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Stress concentration
Shear stress
Matrix Metalloproteinases
Enzymes
Laser Doppler velocimeters
Rubber
Hemodynamics
Silicones
Velocity measurement
Flow fields
Coloring Agents

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

これを引用

Tanoue, T., Tateshima, S., Wakui, D., Vinuela, F., Sudo, R., & Tanishita, K. (2010). Wall shear stress distribution inside induced cerebral aneurysm on rabbit. : IFMBE Proceedings (巻 31 IFMBE, pp. 1063-1066) https://doi.org/10.1007/978-3-642-14515-5_270

Wall shear stress distribution inside induced cerebral aneurysm on rabbit. / Tanoue, T.; Tateshima, S.; Wakui, D.; Vinuela, F.; Sudo, R.; Tanishita, K.

IFMBE Proceedings. 巻 31 IFMBE 2010. p. 1063-1066.

研究成果: Conference contribution

Tanoue, T, Tateshima, S, Wakui, D, Vinuela, F, Sudo, R & Tanishita, K 2010, Wall shear stress distribution inside induced cerebral aneurysm on rabbit. : IFMBE Proceedings. 巻. 31 IFMBE, pp. 1063-1066, 6th World Congress of Biomechanics, WCB 2010 - In Conjunction with 14th International Conference on Biomedical Engineering, ICBME and 5th Asia Pacific Conference on Biomechanics, APBiomech, Singapore, 10/8/1. https://doi.org/10.1007/978-3-642-14515-5_270
Tanoue T, Tateshima S, Wakui D, Vinuela F, Sudo R, Tanishita K. Wall shear stress distribution inside induced cerebral aneurysm on rabbit. : IFMBE Proceedings. 巻 31 IFMBE. 2010. p. 1063-1066 https://doi.org/10.1007/978-3-642-14515-5_270
Tanoue, T. ; Tateshima, S. ; Wakui, D. ; Vinuela, F. ; Sudo, R. ; Tanishita, K. / Wall shear stress distribution inside induced cerebral aneurysm on rabbit. IFMBE Proceedings. 巻 31 IFMBE 2010. pp. 1063-1066
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AU - Tanoue, T.

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AU - Tanishita, K.

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AB - Introduction: Cerebral aneurysm is a cerebrovascular disease resulting to subarachnoid hemorrhage. The generation, development and finally rupture of cerebral aneurysm could be related to hemodynamic stimulate, especially wall shear stress (WSS). WSS have been considered to play a role on the expression of such enzymes as iNOS, MMP-2 and MMP-9 leading to weakening of the arterial wall. However, detailed WSS distribution contributing to the enzyme expression has not been clarified inside cerebral artery. The purpose of our study is to clarify the WSS distribution corresponding to the enzyme expressions. Materials and Methods: We tried to induce cerebral aneurysm on rabbits, and created the realistic silicone model of the induced aneurysm. The flow field and WSS were measured using PIV (particle image velocimetry) and LDV (laser Doppler velocimeter) inside the realistic model. Furthermore, histological structure and enzyme expression were evaluated on the image of elastica van Gieson stain and on the immunostaining images. Results: Induced cerebral aneurysms were found in posterior circulation; the arterial bifurcation at basilar artery or posterior communicating artery. The induced cerebral aneurysm had an arterial wall structure with thinned media and lacked IEL. The thinned media was observed at the bulge top area wth low WSS distribution. On the other hand, lacked IEL was observed near the aneurysm neck area. Although WSS was not so low at the area of lacked IEL, the WSS spatial gradient appeared with high value at this area. The lack of occurs before the thinning of media on the process of aneurysm formation. In addition, MMP-2 and MMP-9 showed similar trend as the WSS spatial gradient. From these findings, high WSS gradient seems to have an effect on the early stage of cerebral aneurysm generation, stimulating the enzymes expression which leads to the destruction of the IEL.

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