Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection

Makoto Kuroda, Atsushi Yamashita, Hideki Hirakawa, Miyuki Kumano, Kazuya Morikawa, Masato Higashide, Atsushi Maruyama, Yumiko Inose, Kimio Matoba, Hidehiro Toh, Satoru Kuhara, Masahira Hattori, Toshiko Ohta

研究成果: Article

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Staphylococcus saprophyticus is a uropathogenic Staphylococcus frequently isolated from young female outpatients presenting with uncomplicated urinary tract infections. We sequenced the whole genome of S. saprophyticus type strain ATCC 15305, which harbors a circular chromosome of 2,516,575 bp with 2,446 ORFs and two plasmids. Comparative genomic analyses with the strains of two other species, Staphylococcus aureus and Staphylococcus epidermidis, as well as experimental data, revealed the following characteristics of the S. saprophyticus genome. S. saprophyticus does not possess any virulence factors found in S. aureus, such as coagulase, enterotoxins, exoenzymes, and extracellular matrix-binding proteins, although it does have a remarkable paralog expansion of transport systems related to highly variable ion contents in the urinary environment. A further unique feature is that only a single ORF is predictable as a cell wall-anchored protein, and it shows positive hemagglutination and adherence to human bladder cell associated with initial colonization in the urinary tract. It also shows significantly high urease activity in S. saprophyticus. The uropathogenicity of S. saprophyticus can be attributed to its genome that is needed for its survival in the human urinary tract by means of novel cell wall-anchored adhesin and redundant uro-adaptive transport systems, together with urease.

元の言語English
ページ(範囲)13272-13277
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
102
発行部数37
DOI
出版物ステータスPublished - 2005 9 13
外部発表Yes

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ASJC Scopus subject areas

  • Genetics
  • General

これを引用

Kuroda, M., Yamashita, A., Hirakawa, H., Kumano, M., Morikawa, K., Higashide, M., Maruyama, A., Inose, Y., Matoba, K., Toh, H., Kuhara, S., Hattori, M., & Ohta, T. (2005). Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection. Proceedings of the National Academy of Sciences of the United States of America, 102(37), 13272-13277. https://doi.org/10.1073/pnas.0502950102