Widefield real-time single-cell secretion imaging with optical waveguide technique

Yumiko Tanaka, Nobutake Suzuki, Kazuyo Mora, Jun Mizuno, Shuichi Shoji, Sotaro Uemura, Yoshitaka Shirasaki*

*この研究の対応する著者

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We report the novel methodology which enables us to analyze single-cell secretion dynamics on the widefield total internal reflection fluorescence imaging with high spatial and temporal resolution using the optical waveguide technique. The practical system was achieved after some sensitivity improvements in an optical waveguide imaging, which has succeeded in detecting very weak fluorescence signals reflecting single-cell secretion dynamics in spite of using a low magnification objective lens. Our methodology is more advantageous than other traditional microscopic technologies in real-time imaging of cell-to-cell communication through humoral factor secretion among actively migrating cells, like immune cells do in vivo.

本文言語English
ホスト出版物のタイトルTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
出版社Institute of Electrical and Electronics Engineers Inc.
ページ1580-1583
ページ数4
ISBN(電子版)9781538627310
DOI
出版ステータスPublished - 2017 7 26
イベント19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
継続期間: 2017 6 182017 6 22

出版物シリーズ

名前TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
国/地域Taiwan, Province of China
CityKaohsiung
Period17/6/1817/6/22

ASJC Scopus subject areas

  • 化学的な安全衛生
  • 器械工学
  • 電子工学および電気工学

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