Yeast-based ligand assay system for detecting G protein-coupled receptor activation in water-in-oil droplets

Takashi Sakurai, Ryo Iizuka, Yasuyuki Tanigaki, Rui Sekine, Dong Hyun Yoon, Tetsushi Sekiguchi, Jun Ishii, Akihiko Kondo, Naoto Nemoto, Shuichi Shoji, Takashi Funatsu

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

We present a yeast-based ligand assay system to detect activation of G protein-coupled receptors (GPCRs) in water-in-oil (W/O) droplets. A microfluidic device is used to encapsulate template DNA encoding peptides, a cell-free coupled transcription-translation system, and budding yeast cells expressing GPCR into W/O droplets. The yeast cells are genetically engineered to express green fluorescent protein (GFP) via their intracellular signaling pathway in response to ligand stimulation of GPCR. We successfully demonstrated that the synthesized peptide ligand activated the cognate GPCR in W/O droplets. This will be a new platform for identifying novel peptide agonists for mammalian GPCRs.

本文言語English
ホスト出版物のタイトルProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
出版社Chemical and Biological Microsystems Society
ページ1537-1539
ページ数3
ISBN(印刷版)9780979806452
出版ステータスPublished - 2012 1 1
イベント16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
継続期間: 2012 10 282012 11 1

出版物シリーズ

名前Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period12/10/2812/11/1

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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