@article{cf3644178641413d827fff26b6298045,
title = "Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako",
abstract = "An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.",
keywords = "Colorimetry, thio-NAD cycling, ultrasensitive ELISA, zeptomole",
author = "Kanako Iha and Yuta Kyosei and Mayuri Namba and Daiki Makioka and Sou Yamura and Satoshi Watabe and Teruki Yoshimura and Etsuro Ito",
note = "Funding Information: This work was partly supported by the Waseda University grants for Specific Research Projects (grant numbers 2017A-015 and 2019C-123). Publisher Copyright: {\textcopyright} 2021. The Japan Society for Analytical Chemistry",
year = "2021",
doi = "10.2116/analsci.21N009",
language = "English",
volume = "37",
pages = "1469--1472",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Japan Society for Analytical Chemistry",
number = "10",
}