Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako

Kanako Iha, Yuta Kyosei, Mayuri Namba, Daiki Makioka, Sou Yamura, Satoshi Watabe, Teruki Yoshimura, Etsuro Ito*

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.

本文言語English
ページ(範囲)1469-1472
ページ数4
ジャーナルAnalytical Sciences
37
10
DOI
出版ステータスPublished - 2021

ASJC Scopus subject areas

  • 分析化学

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